lncRNA LEF1-AS1 Acts as a Novel Biomarker and Promotes Hypopharyngeal Squamous Cell Carcinoma Progression and Metastasis by Targeting the miR-221-5p/GJA1 Axis

<div>miR-221-5p suppresses FaDu cell proliferation and EMT by targeting GJA1. (a) Efficiency of miR-221-5p mimics and inhibitors. (b) Presentation of binding sites between miR-221-5p and GJA1 by TargetScan online tool. (c) Luciferase activity of GJA1-3<svg height="11.4859pt" id="M26" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>UTR-WT or GJA1-3<svg height="11.4859pt" id="M27" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>UTR-mutant plasmid in miR-221-5p overexpressed FaDu cells. (d, e) mRNA (d) or protein (e) levels of GJA1 in miR-221-5p overexpressed or silenced FaDu cells. (f) CCK8 results indicated that the GJA1 overexpression reversed miR-221-5p-induced inhibitory effect on cell proliferation. (g) Western blot results revealed that GJA1 silencing abolished miR-221-5p inhibitor-induced aggravation of EMT. (h) In transwell assays, the GJA1 overexpression reversed miR-221-5p-induced inhibitory effect on FaDu cell migration and invasion. 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Disease Markers

fig5

Figure 5

Figure 5: lncRNA LEF1-AS1 Acts as a Novel Biomarker and Promotes Hypopharyngeal Squamous Cell Carcinoma Progression and Metastasis by Targeting the miR-221-5p/GJA1 Axis 

Figure 5 | lncRNA LEF1-AS1 Acts as a Novel Biomarker and Promotes Hypopharyngeal Squamous Cell Carcinoma Progression and Metastasis by Targeting the miR-221-5p/GJA1 Axis 