[Retracted] Calycosin Inhibits the Malignant Behaviors of Lung Adenocarcinoma Cells by Regulating the circ_0001946/miR-21/GPD1L/HIF-1α Signaling Axis

Figure 3

circ_0001946 can serve as a sponge for miR-21. (a, b) The effect of different concentrations of calycosin on the expression of miR-21 in A549 (a) and H1299 (b) detected by qRT-PCR. (c) The expression of miR-21 in clinical tissue samples (LUAD and normal tissues) detected by qRT-PCR. (d) The correlation between the expression of circ_0001946 and miR-21 in clinical LUAD tissues analyzed by Pearson analysis. (e) Prediction of the targeting sequence of miR-21 and GPD1L. (f) The targeting relationship between circ_0001946 and miR-21 validated by dual-luciferase assay. (g, h) The binding of circ_0001946 to miR-21 in A549 (g) and H1299 (h) cells assessed by RNA pull-down assay. (i, j) The knockdown efficiency of si-circ0001946 on circ_0001946 in A549 (i) and H1299 (j) cells. (k, l) miR-21 expression in A549 (k) and H1299 (l) after knockdown of circ_0001946 detected by qRT-PCR, vs. control.

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