[Retracted] Calycosin Inhibits the Malignant Behaviors of Lung Adenocarcinoma Cells by Regulating the circ_0001946/miR-21/GPD1L/HIF-1α Signaling Axis

Figure 4

miR-21 can target GPD1L. (a, b) The effect of different concentrations of calycosin on GPD1L expression in A549 and H1299 cells observed via qRT-PCR, vs. control. (c) Targeting sequence prediction of miR-21 and GPD1L. (d) The target relationship between miR-21 and GPD1L verified by dual-luciferase assay, vs. GPD1LMUT. (e) The correlation between miR-21 and GPD1L expression in LUAD tissues analyzed by Pearson analysis. (f, g) The knockdown efficiency of miR-21 inhibitor on miR-21 expression in A549 (f) and H1299 (g) cells assessed by qRT-PCR, vs. inhibitor. (h, i) The expression of GPD1L in A549 and H1299 cells after knockdown of miR-21 detected by qRT-PCR, vs. inhibitor. (j, k) The effect of knockdown of miR-21 on the expression of GPD1L in A549 and H1299 cells detected by western blot. vs. inhibitor.

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