Research Article

[Retracted] Calycosin Inhibits the Malignant Behaviors of Lung Adenocarcinoma Cells by Regulating the circ_0001946/miR-21/GPD1L/HIF-1α Signaling Axis

Figure 6

Calycosin regulates colony formation, invasion, migration, and EMT progression of LUAD cells through the circ0001946/miR-21/GPD1L/HIF-1α signaling axis. (a, b) The colony formation ability of A549 cells treated differently assessed by cell colony formation assay. (c, d) The invasion ability of A549 cells in each group detected by transwell. (e, f) The migration ability of A549 cells in each group determined by scratch assay. (g, h) The relative expression of EMT-related proteins (E-cadherin, N-cadherin, and Snail) of A549 cells in each group detected by western blot. (i, j) The mRNA expression of GPD1L (i) and HIF-1α (j) of A549 cells in each group determined by qRT-PCR. (k, l) The protein expression of HIF-1α of A549 cells in each group observed by western blot, vs. control; ## and #vs. CA.
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