Deguelin Attenuates Non-Small-Cell Lung Cancer Cell Metastasis by Upregulating PTEN/KLF4/EMT Signaling Pathway

Figure 4

PTEN upregulates KLF4 expression in NSCLC cells. (a) The expression levels of PTEN in A549 cells transfected with KLF4-siR/overexpression were detected by immunoblotting assay. (b) The expression levels of KLF4 in A549 cells transfected with PTEN-siR/overexpression were detected by immunoblotting assay. (c) The expression levels of PTEN in PC9 cells transfected with KLF4-siR/overexpression were detected by immunoblotting assay. (d) The expression levels of KLF4 in PC9 cells transfected with PTEN-siR/overexpression were detected by immunoblotting assay. β-Actin was used as an internal control. All experiments were repeated at least in triplicate.