PIAS1 Alleviates Hepatic Ischemia-Reperfusion Injury in Mice through a Mechanism Involving NFATc1 SUMOylation

Figure 3

The effect of NFATc1 on HDAC1 expression and inflammatory response and apoptosis in H/R-exposed hepatocytes. (a) The expression of HDAC1 in the liver tissues of sham-operated and HIRI mice determined by RT-qPCR (). (b) The protein expression of HDAC1 in the liver tissues of sham-operated and HIRI mice determined by western blot analysis (). (c) The binding relationship between NFATc1 and HDAC1 in HEK-293 T cells transduced with oe-NFATc1 verified by dual luciferase reporter gene assay (). (d) The expression of HDAC1 in AML12 cells transduced with oe-NFATc1 or sh-NFATc1 determined by RT-qPCR ( vs. oe-NC, # vs. sh-NC). (e) The silencing efficiency of HDAC1 in AML12 cells determined by RT-qPCR (vs. sh-NC). (f) RT-qPCR detection of NFATc1 and HDAC1 expression in AML12 cells transduced with oe-NFATc1 or combined with sh-HDAC1. (g) The mRNA expression of TNF-α, IL-1β, IL-6, Bax, Bcl2, and caspase-3 in AML12 cells transduced with oe-NFATc1 or combined with sh-HDAC1 determined by RT-qPCR. (h) The protein expression of TNF-α, IL-1β, IL-6, Bax, Bcl2. and cleaved caspase-3 in AML12 cells transduced with oe-NFATc1 or combined with sh-HDAC1 determined by western blot analysis. (i) AML12 cell apoptosis following transduction with oe-NFATc1 or combined with sh-HDAC1 detected by TUNEL staining. In (f)–(i), vs. oe-NC + sh-NC, # vs. oe-NFATc1 + sh-NC. All cell experiments were repeated 3 times.

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