PIAS1 Alleviates Hepatic Ischemia-Reperfusion Injury in Mice through a Mechanism Involving NFATc1 SUMOylation

Figure 4

The effect of HDAC1 on IRF-1 transcription and hepatocyte inflammatory response and apoptosis in H/R-exposed hepatocytes. (a) The mRNA expression of IRF-1 in the liver tissues of sham-operated and HIRI mice determined by RT-qPCR (). (b) The protein expression of IRF-1 in the liver tissues of sham-operated and HIRI mice determined by western blot analysis (). (c) The binding between HDAC1 and IRF-1 in HEK-293 T cells transduced with oe-HDAC1 determined by dual luciferase reporter gene assay (). (d) The expression of IRF-1 in AML12 cells transduced with oe-HDAC1 or sh-HDAC1 determined by RT-qPCR ( vs. oe-NC, # vs. sh-NC). (e) The silencing efficiency of IRF-1 in AML12 cells determined by RT-qPCR ( vs. sh-NC). (f) RT-qPCR detection of HDAC1 and IRF-1 expression in H/R-exposed AML12 cells transduced with oe-HDAC1 or combined with sh-IRF-1. (g) The mRNA expression of TNF-α, IL-1β, IL-6, Bax, Bcl2, and caspase-3 in H/R-exposed AML12 cells transduced with oe-HDAC1 or combined with sh-IRF-1 determined by RT-qPCR. (h) The protein expression of TNF-α, IL-1β, IL-6, Bax, Bcl2 ,and cleaved caspase-3 in H/R-exposed AML12 cells transduced with oe-HDAC1 or combined with sh-IRF-1 determined by western blot analysis. (i) Apoptosis of H/R-exposed AML12 cells following transduction with oe-HDAC1 or combined with sh-IRF-1 determined by TUNEL staining. In (f)–(i), vs. oe-NC + sh-NC, # vs. oe-HDAC1 + sh-NC. All cell experiments were repeated 3 times.

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