PIAS1 Alleviates Hepatic Ischemia-Reperfusion Injury in Mice through a Mechanism Involving NFATc1 SUMOylation

Figure 6

Overexpression of PIAS1 inactivated the NFATc1/HDAC1/IRF-1/p38 MAPK pathway to alleviate HIRI in mice. HIRI mice were treated with oe-PIAS1 or combined with anisomycin (p38 MAPK activator). (a) The protein expression of PIAS1, NFATc1, HDAC1, IRF-1, and phosphorylation levels of p38 in the liver tissue of mice determined by western blot analysis. (b) The serum ALT activity in mice determined by ELISA. (c) The serum AST activity in mice determined by ELISA. (d) The degree of liver injury in mice determined by H&E staining. (e) The degree of liver injury in mice determined by MDA detection. (f) The neutrophil activity in the liver tissue of mice determined by MPO detection. (g) The expression of TNF-α, IL-1β, and IL-6 levels in the liver tissue of mice determined by ELISA. (h) The levels of Gr-1 and CD68 in liver tissues of mice determined by immunofluorescence staining. (i) Hepatocyte apoptosis in liver tissues of mice determined by TUNEL assay. , vs. sham-operated mice, # vs. HIRI + oe-NC, & vs. HIRI + oe-PIAS1.

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