Liraglutide Alleviates Diabetic Atherosclerosis through Regulating Calcification of Vascular Smooth Muscle Cells

Figure 3

LIRA inhibits the ALP activity of VSMC through the PI3K/AKT and ERK1/2 MAPK signaling pathways. (a) Cell identification by the cell morphology under light microscope (100x) and SMα-actin immunofluorescence (400x). (b) ALP activity detection. Data from three independent experiments are expressed as deviation; vs. control; vs. HG; vs. HG + LIRA. (c) Protein expression of phosphorylated Akt in VSMC. (d) Protein expression of phosphorylated ERK1/2 in VSMC. Data from three independent experiments are expressed as deviation; vs. control; vs. HG; , vs. HG + LIRA.

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