Association of Clinical Features of Colorectal Cancer with Circulating Tumor Cells and Systemic Inflammatory Markers

<div>EMT phenotypes of CTCs and white blood cells (WBCs) were detected by RNA in situ hybridization in CRC patients. Cell nuclei were stained with DAPI (blue). White blood cells (WBCs) were detected using CD45 as the marker (white). Epithelial circulating tumor cells (E<sup>+</sup> CTCs) were detected using EpCAM and CK8/18/19 as markers (red); mesenchymal circulating tumor cells (M<sup>+</sup> CTCs) were detected with vimentin and twist as markers (green) dots; E<sup>+</sup>/M<sup>+</sup> CTCs exhibited both epithelial and mesenchymal markers (red and green) (Under ×10 eyepiece and ×40 objective magnification. <span class="nowrap"><svg height="12.7373pt" id="M8" style="vertical-align:-3.429399pt" version="1.1" viewbox="-0.0498162 -9.3079 87.2791 12.7373" width="87.2791pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M409 504C401 567 396 607 392 642C354 654 312 665 266 665C137 665 60 583 60 487C60 374 161 325 225 290C300 250 355 215 355 141C355 68 311 21 235 21C131 21 86 122 71 183L41 176C48 128 61 42 68 21C78 16 93 8 118 0C142 -7 175 -15 216 -15C349 -15 438 69 438 174C438 287 344 333 265 374C186 414 138 449 138 522C138 576 172 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Disease Markers

fig1

Figure 1

Figure 1: Association of Clinical Features of Colorectal Cancer with Circulating Tumor Cells and Systemic Inflammatory Markers 