Protein Tyrosine Phosphatase Receptor Type R (PTPRR) Reduces AChR Clustering by Dephosphorylating MuSK

<div>MuSK is an essential molecule that mediates AChR clustering. (a) The efficiency of <i>MuSK</i> knockout (<i>MuSK<sup>−</sup></i>) was validated by western blot. MuSK<sup>−</sup> single clone C2C12 generated by CRISPR sgRNA2 was chosen for the following experiments. (b) Almost no clusters of AChRs were formed in <i>MuSK</i><sup>−</sup> C2C12 myotubes compared with wild-type myotubes. White arrowheads indicate AChR clusters. (c) Quantification of AChR clusters ≥5 <i>μ</i>m in length from (b). The data were obtained from at least three experiments. 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Disease Markers

fig2

Figure 2

Figure 2: Protein Tyrosine Phosphatase Receptor Type R (PTPRR) Reduces AChR Clustering by Dephosphorylating MuSK 