Protein Tyrosine Phosphatase Receptor Type R (PTPRR) Reduces AChR Clustering by Dephosphorylating MuSK

<div>PTPRR was identified as the tyrosine phosphatases responsible for dephosphorylating MuSK via a protein tyrosine phosphatases screening. (a) HEK293T cells were cotransfected with flag-MuSK and 10 various tyrosine phosphatases as indicated. Tyrosine phosphorylation of MuSK and actin were probed. (b) HEK293T cells were transiently transfected with MuSK, PTPRR-WT, or PTPRR-DA mutant. MuSK was isolated by immunoprecipitation with anti-Flag antibody, probed with 4G10 to reveal pY-MuSK, and probed with PTPRR to reveal the interaction between MuSK and PTPRR. Lysates were probed with antibodies against MuSK, PTPRR, and <i>β</i>-actin. The data were obtained from at least three experiments. 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Disease Markers

fig3

Figure 3

Figure 3: Protein Tyrosine Phosphatase Receptor Type R (PTPRR) Reduces AChR Clustering by Dephosphorylating MuSK 