Research Article
Crosslinking-Induced Corneal Endothelium Dysfunction and Its Protection by Topical Ripasudil Treatment
Figure 6
Ripasudil reduces corneal edema and exhibits protection of the corneal endothelium from CXL irradiation in mice. (a) Mouse corneal clarity was examined by slit-lamp microscopy with or without ripasudil treatment after 7 days of CXL treatment. (b) Representative OCT images of mouse corneas after 7 days of CXL treatment. (c) Representative in vivo confocal images of corneal endothelial cells after 7 days of CXL treatment. (d) Analysis of the corneal thickness based on the OCT image on day 7 after CXL irradiation. (e) Endothelial cell density analysis of corneas on day 7 after CXL irradiation. (f) Representative confocal images of wholemount mouse corneal endothelium with ZO-1, Na+/K+-ATPase, and γ-H2AX staining on day 7 after CXL irradiation. Data are expressed as the ; . . U(-), R(-), Ripa(-): no UVA, riboflavin, or ripasudil treatment; U(++), R(+), Ripa(-): 1.08 J/cm2 UVA plus riboflavin only; U(++), R(+), Ripa(+): 1.08 J/cm2 UVA, riboflavin, and ripasudil treatment.
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