Cell-Free DNA Variables including Gene Mutations in CA15-3 Normal Breast Cancer Reflect Prognosis

Table 5

Logistic regression analysis between cfDNA variables and metastatic status in BC patients with normal CA15-3 levels.

Characteristics	Univariable analysis			Multivariate analysis
	OR	95% CI		OR	95% CI
Age at diagnosis (year)
≤50	1.000			1.000
>50	0.387	0.145-1.030	0.057	0.316	0.098-1.018	0.054
Tumor size (cm)
<2	1.000
≥2	1.572	0.698-3.537	0.275
Stage
I/II	1.000			1.000
III/IV	0.840	0.317-2.228	0.726	0.813	0.247-2.678	0.733
ER/PR/HER2 status at diagnosis
TNBC	1.000
HER2+ (any ER/PR)	0.500	0.068-3.675	0.497
HER2- (ER or/and PR+)	0.389	0.065-2.331	0.301
cfDNA concentration
Low	1.000			1.000
High	4.400	1.588-12.193	0.004	3.404	1.074-10.788	0.037
cfDNA integrity
Low	1.000
High	0.489	0.186-1.286	0.147
CNV
Low	1.000
High	2.046	0.777-5.386	0.147
SNV
No	1.000			1.000
Yes	3.818	1.396-10.443	0.009	2.088	0.583-7.476	0.258
TP53 mutation
No	1.000			1.000
Yes	8.905	1.795-44.186	0.007	4.536	0.730-28.206	0.105

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