Cell-Free DNA Variables including Gene Mutations in CA15-3 Normal Breast Cancer Reflect Prognosis

Table 6

Logistic regression analysis between cfDNA variables and distant metastatic status in BC patients with normal CA15-3 levels.

Characteristics	Univariable analysis			Multivariate analysis
	OR	95% CI		OR	95% CI
Age at diagnosis (year)
≤50	1.000			1.000
>50	1.543	0.448-5.311	0.492	2.627	0.467-14.782	0.273
Tumor size (cm)
<2	1.000
≥2	2.289	0.824-6.355	0.112
Stage
I/II	1.000			1.000
III/IV	1.388	0.410-4.694	0.598	0.862	0.169-4.382	0.858
ER/PR/HER2 status at diagnosis
TNBC	1.000
HER2+ (any ER/PR)	0.800	0.102-6.249	0.832
HER2- (ER or/and PR+)	0.341	0.052-2.231	0.262
cfDNA concentration
Low	1.000			1.000
High	18.000	2.182-148.486	0.007	13.750	1.473-128.358	0.021
cfDNA integrity
Low	1.000			1.000
High	0.232	0.058-0.938	0.040	0.285	0.055-1.462	0.132
CNV
Low	1.000			1.000
High	4.306	1.066-17.389	0.040	4.192	0.718-24.494	0.112
SNV
No	1.000			1.000
Yes	4.638	1.147-18.751	0.031	1.363	0.201-9.233	0.751
TP53 mutation
No	1.000			1.000
Yes	5.878	1.526-22.633	0.010	4.154	0.596-28.951	0.151

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