Extracellular Vesicles Carrying miR-887-3p Promote Breast Cancer Cell Drug Resistance by Targeting BTBD7 and Activating the Notch1/Hes1 Signaling Pathway

Figure 3

MDA-MB-231-derived EVs carried miR-887-3p into BC cells. ECORI Pan-Cer database analyzed the expression (a) and prognosis (b) of miR-887-3p in BC. EVs were treated with RNase (RNase-group) and RNase+SDS (RNase+SDS-group), respectively. miR-887-3p expression was detected using RT-qPCR (c). Next, miR-Inhibitor was transfected into MDA-MB-231 cells while Mock miR served as a negative control. Then, EVs (EV-NC group and EV-inhi group) were extracted as described in a method. RT-qPCR was performed to determine miR-887-3p expression in MDA-MB-231 cells (d) and EVs (e). MCF-7, BT474, and HCC1937 cells were treated with EVs in different groups, and then RT-qPCR was performed to determine miR-887-3p expression (f). Data were expressed as the . In (f), two-way ANOVA and Tukey’s multiple comparison test were used to determine statistical significance, whereas in (c)–(e) one-way ANOVA and Tukey’s multiple comparison test were used. . Three independent experiments were performed. BC: breast cancer; miR: microRNA; RT-qPCR: reverse transcription quantitative polymerase chain reaction; ANOVA: analysis of variance.

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