Extracellular Vesicles Carrying miR-887-3p Promote Breast Cancer Cell Drug Resistance by Targeting BTBD7 and Activating the Notch1/Hes1 Signaling Pathway

Figure 6

MDA-MB-231-derived EVs carrying miR-887-3p targeted BTBD7 and activated Notch1/Hes1 signaling pathway to promote BC cell drug resistance. MCF-7, HCC1937, and BT474 cells were treated with EVs and transfected with oe-BTBD7. (a) RT-qPCR was performed to determine BTBD7 mRNA expression. CCK-8 assay (b), colony formation assay (c), and flow cytometry (d) were performed to determine the effects of BTBD7 on BC cell drug resistance. Western blot analysis (e) was performed to determine Notch1 and Hes1 protein level in MCF-7, BT474, and HCC1937 cells under MDA-MB-231-derived EV treatments. Data are expressed as the . One-way ANOVA and Tukey’s multiple comparison test were used. . Three independent experiments were performed. BC: breast cancer; miR: microRNA; BTBD7: BTB domain containing 7; CCK-8: cell counting kit-8; RT-qPCR: reverse transcription quantitative polymerase chain reaction; ANOVA: analysis of variance.

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