KLF4 Affects Acute Renal Allograft Injury via Binding to MicroRNA-155-5p Promoter to Regulate ERRFI1

Figure 1

KLF4 and miR-155-5p are overexpressed in mice with acute renal allograft injury. (a) Serum creatinine and eGFR contents in mice. (b) ELISA detected serum IFN-γ, TNF-α, and IL-2 contents in mice. (c) H&E staining detected kidney injury of mice. (d) TUNEL staining detected renal tubular apoptosis of mice. (e) Western blot detected Bax and Bcl-2 protein expression in renal tissues of mice. (f) MDA content and SOD activity in renal tissues of mice. (g) RT-qPCR detected KLF4 and miR-155-5p expressions in renal tissues of mice. (h) CCK-8 assay detected proliferation of H/R-treated HK-2 cells. (i) Flow cytometry detected the apoptosis rate of H/R-treated HK-2 cells. (j) Western blot detected Bax and Bcl-2 protein expressions in H/R-treated HK-2 cells. (k) RT-qPCR detected KLF4 and miR-155-5p expressions in H/R-treated HK-2 cells. The data were expressed as ; compared with the sham/blank group. (a–g) and (h–k) .

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