ABO rs657152 and Blood Groups Are as Predictor Factors of COVID-19 Mortality in the Iranian Population

Table 1

Sequences and melting temperatures of the ABO rs657152 primers.

SNP	Primer	Primer sequence	Size, bp	Tm	Annealing temp
ABO rs657152 (A/C)	OF	5-ACTACTTCATCTGTTACTTCTTATCTTAT-3	—	61.0°C	55.0°C
	OR	5-GTGTGAAACTCATCAAAACCGTATA-3	339	61.0°C
	IF	5-TATCTCGAATAGCTTCTTGAAGCC-3	255	61.8°C
	IR	5-TTGCCTCCCACGTTCCT-3	124	62.0°C

The specific nucleotides at 3-end of primers are in bold, while the mismatches are underlined. SNP: single nucleotide polymorphism; OF: outer forward primer; OR: outer reverse primer; IF: inner forward primer; IR: inner reverse primer.