[Retracted] circCNN2 Accelerates Cell Proliferation and Invasion in Lung Squamous Cell Carcinoma via Regulating miR-184/E2F1 and Activating MAPK Signaling Pathway

Figure 4

circCNN2 functions as a ceRNA by competitively sponging miR-184. (a, b) RIP assay with gel electrophoresis was conducted to detect the enrichment of circCNN2 in RNA-induced silencing complex (RISC). (c) RT-qPCR was used to examine the expression of miR-184 in H1703 and H226 cells compared with Beas-2B cell line. (d) RIP assay was carried out to evaluate miR-184 enrichment in anti-AGO2 and anti-IgG groups. (e) RNA pull down assay was adopted to test the enrichment of miR-184 in Bio-circCNN2-Wt and Bio-circCNN2-Wut groups. (f) Luciferase report assays were conducted to evaluate the luciferase activity of wild-type or mutant circCNN2 plasmids upon mimics NC and miR-184 mimics. (g) RT-qPCR was conducted to examine miR-184 expression in H1703 and H226 cells after inhibiting circCNN2. compared to the control group.

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