Glaucocalyxin A Attenuates IL-1β-Induced Inflammatory Response and Cartilage Degradation in Osteoarthritis Chondrocytes via Inhibiting the Activation of NF-κB Signaling Pathway

Figure 4

Modulation of TNF-α, IL-6, and IL-8 production by GLA in human OA chondrocytes. Primary human OA chondrocytes were incubated with different concentrations of GLA (0, 5, 10, and 20 μM) for 2 h and then stimulated with IL-1β (10 ng/ml) for 24 h. (a–c) The mRNA levels of TNF-α, IL-6, and IL-8 were evaluated using qRT-PCR. (d–f) The contents of TNF-α, IL-6, and IL-8 in culture supernatant were determined using ELISA. versus the control group; # versus the IL-1β group.

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