Urolithin B, a Gut Microbiota Metabolite, Reduced Susceptibility to Myocardial Arrhythmic Predisposition after Hypoxia

Figure 2

Hypoxia stimulates cardiomyocyte apoptosis and alters gap junction and neural remodeling levels in vitro. (a) TUNEL immunofluorescence staining in cardiomyocytes in the hypoxia group and control group (). (b) Quantification of the percentage of TUNEL-positive cardiomyocytes in the hypoxia group and control group. ; per group (Student’s -test). (c–e) Q-PCR assay detecting the expression levels of bcl2, bax, and caspase-3 in the hypoxia group and control group. (f) WB assay detecting the expression levels of bcl2, bax, and caspase-3 in the hypoxia group and control group. (g) Quantification of bcl2, bax, and caspase-3 protein levels. ; per group (Student’s -test). (h–k) Q-PCR assay detecting the expression levels of IL-6, CX43, TH, and GAP43 in the hypoxia group and control group. (l–o) Western blotting and densitometric analysis of the protein levels of IL-6, CX43, TH, and GAP43 in the hypoxia group and control group. ; per group (Student’s -test).

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