Urolithin B, a Gut Microbiota Metabolite, Reduced Susceptibility to Myocardial Arrhythmic Predisposition after Hypoxia

Figure 3

Urolithin B reduced myocardial apoptosis and regulated neural remodeling and gap junction channels. (a–c) Q-PCR assay detecting the expression levels of bcl2, bax, and caspase-3 in the hypoxia group and the hypoxia+urolithin B group. (d, e) WB assay detecting the expression levels of bcl2, bax, and caspase-3 in the hypoxia group and the hypoxia+urolithin B group. (f) TUNEL immunofluorescence staining in cardiomyocytes in the hypoxia group and the hypoxia+urolithin B group (). (g) Quantification of the percentage of TUNEL-positive cardiomyocytes in the hypoxia group and the hypoxia+urolithin B group. ; per group (Student’s -test). (h–k) Q-PCR assay detecting the expression levels of IL-6, CX43, TH, and GAP43 in the hypoxia group and the hypoxia+urolithin B group. (l, m) Western blotting and densitometric analysis of the protein levels of IL-6, CX43, TH, and GAP43 in the hypoxia group and control group. ; per group (Student’s -test).

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