Integrated Analysis of the Role of Enolase 2 in Clear Cell Renal Cell Carcinoma

Figure 4

Inhibition of ENO2 can hamper malignant behaviors of ccRCC cell. Notes: (a) The knockdown efficiency of three designed shRNA were evaluated using qPCR assay, with results showing that shENO2#2 was the best. (b–d) CCK8 and colony formation assays indicated that ENO2 could significantly promote proliferation of ccRCC cells. (e) Transwell assay showed that ENO2 significantly promoted invasion and migration of ccRCC cells. (f) In vivo experiments showed that the knockdown of ENO2 remarkably lowered the growth of tumor in mice. (g) Immunohistochemistry showed a higher ENO2-positive staining level in the mice tumor tissue inoculated with sh-NC cells.

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