miR-373-3p Regulates the Proliferative and Migratory Properties of Human HTR8 Cells via SLC38A1 Modulation

Figure 1

HIF-1α knockdown increased HTR8 cell migration and proliferation under hypoxic conditions. (a, b) qRT-PCR and western blot of the relative mRNA and protein levels of HIF-1α under normal and hypoxic conditions in human HTR8 cells. vs Normal. (c, d) HIF-1α shRNAs significantly suppressed HIF-1α expression in HTR8 cells. vs shNC. (e) Transwell assay of cell migration. vs shNC. vs shNC. (f) CCK8 assay of the proliferation of HTR8 cells after transfecting shNC and shHIF-1α under hypoxia conditions. vs control, vs control; vs hypoxia + shNC. (g) qRT-PCR of the relative mRNA levels of SLC38A1 and miR-373-3p in HTR8 cells after transfecting with shNC or shHIF-1α under hypoxic conditions. vs control; vs hypoxia + shNC. (h) Western blot of SLC38A1 protein expression in HTR8 cells after transfecting with shNC or shHIF-1α under hypoxic conditions.

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