miR-373-3p Regulates the Proliferative and Migratory Properties of Human HTR8 Cells via SLC38A1 Modulation

Figure 4

miR-373-3p inhibited SLC38A1 function by binding to its 3-UTR. (a) WT and mutant binding sites of miR-373-3p in the 3-UTR of SLC38A1. (b) Mutant 3-UTR abolished the interaction between miR-373-3p and SLC38A1. vs miNC. (c, d) Lentiviral-mediated vector in HTR8 cells induced SLC38A1 overexpression. vs oeNC. (e) SLC38A1 overexpression significantly upregulated the proliferation of mimic treated cells. vs oeNC + miNC, vs oeNC + miNC, vs oeSLC38A1 + miNC, vs oeSLC38A1 + miNC. (f) The miR-373-3p mimic inhibited the migration of oeSLC38A1 transfected cells. vs oeNC + miNC, vs oeNC + miNC, vs oeNC + miNC. vs oeSLC38A1 + miNC, vs oeSLC38A1 + miNC. (g) Western blot of SCL38A1, mTOR, and p-mTOR protein levels in cells.

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