miR-602 Activates NRF2 Antioxidant Pathways to Protect HBMECs from OGD/R-Induced Oxidative Stress via Targeting KEAP1 and HRD1

Figure 6

HRD1 Silence could reserve OGD/R-induced injury to HBMECs at miR-602 deficiency situation. HBMECs were transfected with mimic of miR-602 inhibitor and HRD1 for 48 hours and then subjected to OGD/R treatment. (a) NRF2 and MCM3 relative expression level were detected by Western Blot. (b) NRF2 and HRD1 relative expression level at miR-602 deficiency situation was detected by Western Blot. (c) Luciferase activity of HRD1 3-UTR wt and HRD1 3-UTR mut with miR-602. (d) The effect of HRD1 silence on ROS level of HBMECs with low miR-602 level was detected by ROS detection assay. (e) The effect of HRD1 silence on relative NRF2/ARE transcription activity in HBMECs with low miR-602 level was detected by luciferase reporter assay. (f) The effect of HRD1 silence on the viability of HBMECs with low miR-602 level was detected by CCK-8. .

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