Pygo1 Regulates the Behavior of Human Non-Small-Cell Lung Cancer via the Wnt/β-Catenin Pathway

Figure 2

Overexpression of Pygo1 induced G1/S phase transformation of A549 cells and inhibited apoptosis. (a, b) Pygo1 protein was overexpressed in A549 cell lines and its fold change. EV: blank control; Tag2B: negative control; Pygo1: Pygo1 overexpression cells; GAPDH: loading control (). (c) Quantitative analysis of cell cycle stage by flow cytometry. (d) Quantitative analysis of cell proliferation detected by CCK-8. (e) Quantitative analysis of apoptosis by flow cytometry. (f, g) Cleaved-caspase-3 protein level detected by western blotting and its fold change. (h, i) Cell cycle-related proteins detected by western blotting and their fold change by ImageJ and GraphPad Prism (). ns , , , and .

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