Pygo1 Regulates the Behavior of Human Non-Small-Cell Lung Cancer via the Wnt/β-Catenin Pathway

Figure 4

Pygo1 upregulation in NSCLC was related to the Wnt signaling pathway. (a, b) Western blotting analysis of lysates with different treatments using rabbit polyclonal antibody to β-catenin, GAPDH was used as a loading control (). (c) Representative images of immunohistochemical (IHC) analysis of β-catenin expression levels in NSCLC from different pathological grades and adjacent tissues and (d) quantitative analysis of IHC images using ImageJ. Grade I, grade II, and grade III refer to WHO-IA-LUAD, WHO-IIA-LUAD, and WHO-IIIB-LUSC, respectively. Scale bar: 100 μm. ns , , , and . (e) Coimmunoprecipitation analysis of TCF4 and β-catenin expression in A549 cells. (f, g) Western blotting analysis of Wnt/β-catenin signal proteins and quantitative analysis (); ns , , , and . (h) Necropsy photographs of mice and xenograft tumors on day 41. The results shown are representative of independent experiments. (i) Tumor weight was measured on day 41 of the experiment (). (j, k) Western blot analysis of the tissues samples and quantitative analysis (); ns , , , and . Equal amounts of lysates were analyzed via western blot analysis and then probed for different proteins.

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