Leptin Promotes HTR-8/SVneo Cell Invasion via the Crosstalk between MTA1/WNT and PI3K/AKT Pathways

Figure 2

Leptin induces HTR-8/SVneo cell invasion by promoting MMP9 expression. (a) HTR-8/SVneo cells were treated with exogenous leptin (0, 50, 100, and 200 ng/ml) for 24 h, and MMP9 protein levels were measured by Western blot analysis. Data are shown as ; vs. leptin (0 ng/ml), vs. leptin (50 ng/ml), and vs. leptin (100 ng/ml). (b) Representative immunofluorescence images of MMP9 in HTR-8/SVneo cells treated with exogenous leptin (0, 50, 100, and 200 ng/ml) for 24 h. . (c) The knockdown efficiency of MMP9 was analyzed by Western blot analysis and qRT-PCR. vs. control. (d) The results of transwell assay in HTR-8/SVneo cells treated with MMP9 siRNA or scramble siRNA (Scr) in the presence or absence of 200 ng/ml leptin for 24 h. . Data are shown as ; vs. control, ^# vs. leptin. (e) Results from the wound-healing assay are expressed as the percentage of wound closure. Magnification, 100x. Data are shown as ; vs. control, ^# vs. leptin. All experiments were performed in triplicate.

(a)

(b)

(c)

(d)

(e)