Leptin Promotes HTR-8/SVneo Cell Invasion via the Crosstalk between MTA1/WNT and PI3K/AKT Pathways

Figure 3

Leptin induced β-catenin activation in HTR-8/SVneo cells. (a) HTR-8/SVneo cells were treated with exogenous leptin (200 ng/ml) for 24 h, and the expression of β-catenin from nuclear and cytoplasmic fractions was detected by Western blot. Histone 3 was used as a nuclear marker and β-actin as a cytoplasmic marker. The relative β-catenin nuclear-to-cytoplasmic (N/C) ratio was calculated with nuclear β-catenin (normalized to histone 3) to cytoplasmic β-catenin (normalized to β-actin). Data are shown as ; vs. leptin (0 ng/ml). (b) Representative immunofluorescence images of β-catenin in HTR-8/SVneo cells treated with exogenous leptin (0, 50, 100, and 200 ng/ml) for 24 h. . All experiments were performed in triplicate.

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