Leptin Promotes HTR-8/SVneo Cell Invasion via the Crosstalk between MTA1/WNT and PI3K/AKT Pathways

Figure 5

MTA1/WNT and PI3K/AKT pathways participated in leptin-induced HTR-8/SVneo cell invasion through promoting MMP9 expression. (a) Western blot and immunofluorescence staining of MMP9 levels in HTR-8/SVneo cells treated with β-catenin siRNA or scramble siRNA (Scr) in the absence or in the presence of 200 ng/ml leptin for 24 h. . (b) Western blot and immunofluorescence staining of MMP9 levels in HTR-8/SVneo cells treated with MTA1, AKT, and WNT1 in the absence or in the presence of 200 ng/ml leptin for 24 h. . (c) The results of transwell assay in HTR-8/SVneo cells treated with MTA1 siRNA, AKT siRNA, WNT1 siRNA, β-catenin siRNA, or scramble siRNA (Scr) in the absence or in the presence of 200 ng/ml leptin for 24 h. . Data are shown as ; vs. Control and ^# vs. leptin. (d) Results from the wound-healing assay are expressed as the percentage of wound closure. Magnification, 100x. Data are shown as ; vs. control and ^# vs. leptin. All experiments were performed in triplicate.

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