Exosomes Derived from Adipose Mesenchymal Stem Cells Carrying miRNA-22-3p Promote Schwann Cells Proliferation and Migration through Downregulation of PTEN

Figure 2

Characterization of exosomes from ADSCs. (a) Representative ultrastructure of ADSC-Exos by TEM. (b) The size distribution profile of ADSC-Exos by NTA. (c) Detection of exosomal markers (ALIX and CD9) and GAPDH by western blot.(d) The internalization of ADSC-Exos by SCs. Confocal images showing the SCs incubated with 5, 10, and 20 g/ml PKH67-labeled ADSC-Exos for 24 hour (scale bar : 100 m). TEM, Transmission Electron Microscope; NTA, Nanoparticle Tracking Analysis; DAPI, 4-6-diamidino-2-phenylindole; PKH67, Green Fluorescent Cell Linker; Actin Red, phalloidin-Alexa Fluor 594; ADSCs, Adipose-derived mesenchymal stem cells; ADSC-Exos, the exosomes extracted from Adipose-derived mesenchymal stem cells.

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