Exosomes Derived from Adipose Mesenchymal Stem Cells Carrying miRNA-22-3p Promote Schwann Cells Proliferation and Migration through Downregulation of PTEN

Figure 5

miR-22-3p targets PTEN and activates the AKT/mTOR signaling pathway. (a) The predicted binding sites of miR-22-3p within the 3-UTR of Pten. (b) Dual-luciferase reporter assays showed miRNA-22-3p cotransfected with wild-type or mutation Pten 3-UTR in SCs. (c) Pten mRNA expression in SCs after the treatment with miR-22-3p mimic, miR-22-3p inhibitor, and NC. (d) Effects of miR-22-3p mimic and miR-22-3p inhibitor on the protein expressions of AKT/mTOR pathway compare with control group. Data are expressed as means ± SD. Statistical significance was obtained with unpaired Student’s test or one way ANOVA. means ; NS means no significant. ADSC-Exos, the exosomes extracted from Adipose-derived mesenchymal stem cells; SCs, Schwann cells; miR, miRNA.

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