Phenytoin Is Promoting the Differentiation of Dental Pulp Stem Cells into the Direction of Odontogenesis/Osteogenesis by Activating BMP4/Smad Pathway

Figure 3

CCK-8 experiment results and the expression of odontogenic/osteogenic genes and proteins at different drug concentrations on 14 days. hDPSCs were cultured in general medium containing different concentrations of PHT for 14 days. Proteins and mRNAs were collected on day 14 after induction for Western blot and qRT-PCR. (a) CCK-8 cytotoxicity experiment showed the concentration below 200 mg/L PHT had no obvious toxicity to hDPSC. (b) CCK-8 cell proliferation experiment showed that the inhibitory effect of hDPSC proliferation increased with the higher PHT concentration. (c)–(e) show the expression of DSPP, DMP1, and ALP genes at different drug concentrations on 14 days, and related genes were elevated in the 50 mg/L and 100 mg/L PHT concentration. (f, g) Western Blot experiment showed the protein expression levels of DSPP and DMP1 were significantly upregulated in the 50 mg/L PHT concentration. (h) ALP staining showed an increase in the 50 mg/L PHT group on 14 days.

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