HMGA1 As a Potential Prognostic and Therapeutic Biomarker in Breast Cancer

Figure 5

Knockdown of HMGA1 inhibited the proliferation, apoptosis, migration, and invasion of breast cancer cells. (a) The migration and invasion of MCF-7 was examined by transwell assay. (b) The migration and invasion of MDA-MB-231 was examined by transwell assay. (c) The proliferation of MCF-7/MDA-MB-231 cells was examined by CCK-8 assay. (d) The proliferation of MCF-7/MDA-MB-231 cells was examined by EdU assay. (e) Representative cell cycle images of MCF-7 at 48 h after transfection with 100 nmol siNC or 100 nmol specific siHMGA1 by flow cytometry. (f) Representative cell cycle images of MDA-MB-231 at 48 h after transfection with 100 nmol siNC or 100 nmol specific siHMGA1 by flow cytometry. (g) Representative FACS analyses for the induction of apoptosis in HMGA1 on MCF-7 and MCF-7/si-HMGA1 cells. (h) Representative FACS analyses for the induction of apoptosis in HMGA1 on MDA-MB-231, and MDA-MB-231/si-HMGA1 cells. (i) The expression levels of indicated proteins were evaluated by western blot in control and HMGA1-silenced MCF-7/MDA-MB-231cells. Data were shown as . ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)