Expression, Purification, and In Silico Characterization of <i>Mycobacterium smegmatis</i> Alternative Sigma Factor SigB

<div>Expression host strain optimization for the expression of recombinant His-SigB. 10% SDS PAGE depicting recombinant His-SigB protein expression profile from three expression host strains, namely, BL21(DE3), C41 (DE3), and CodonPlus (DE3) expressed with 0.1 mM IPTG grown for 3 hrs at 37°C with shaking at 150 rpm. The highest protein expression is observed in the host strain CodonPlus (DE3). M: molecular size marker; V.O.: pET28a vector only control; U: uninduced His-SigB clone; I: induced His-SigB clone.</div>

Disease Markers

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Figure 1

Figure 1: Expression, Purification, and In Silico Characterization of <i>Mycobacterium smegmatis</i> Alternative Sigma Factor SigB 

Disease Markers

Expression, Purification, and In Silico Characterization of Mycobacterium smegmatis Alternative Sigma Factor SigB

Figure 1