Expression, Purification, and In Silico Characterization of <i>Mycobacterium smegmatis</i> Alternative Sigma Factor SigB

<div>(a) The expression of recombinant His-SigB protein at different time points. 10% SDS PAGE depicting recombinant His-SigB protein expression profile collected at time points 15, 30, 60, 120, and 180 minutes. The highest protein expression was observed at 180 minutes after induction with 0.1 mM IPTG. (b) Ni-NTA affinity purification of His-SigB: lanes denoting M: molecular size marker; 1: 5 <i>μ</i>g; 2: 7.5 <i>μ</i>g His-SigB purified protein stained with Coomassie R-250. (c) Western blotting with purified recombinant His-SigB. Mouse anti-His monoclonal antibody (Genetix) was used to probe purified His-SigB. M: marker; V.O.: pET28a vector only control; U: uninduced His-SigB clone; lane 1: 5 <i>μ</i>g; lane 2: 10 <i>μ</i>g purified His-SigB protein.</div>

Disease Markers

fig4

Figure 4

Figure 4: Expression, Purification, and In Silico Characterization of <i>Mycobacterium smegmatis</i> Alternative Sigma Factor SigB 

Disease Markers

Expression, Purification, and In Silico Characterization of Mycobacterium smegmatis Alternative Sigma Factor SigB

Figure 4