Thyroid-Stimulating Hormone Inhibits Insulin Receptor Substrate-1 Expression and Tyrosyl Phosphorylation in 3T3-L1 Adipocytes by Increasing NF-<i>κ</i>B DNA-Binding Activity

<div>Effect of TSH on TNF-<i>α</i>-induced inhibition of insulin signals in 3T3-L1 adipocytes. Adipocytes were treated with 100 nM insulin and then coincubated with 1 mIU/ml TSH in the absence or presence of WP9QY. (a) Cells treated with insulin (the positive control group) exhibited higher IRS-1 tyrosyl phosphorylation levels than untreated control cells (the negative control group). TSH decreased IRS-1 tyrosyl phosphorylation. (b) Pretreatment with WP9QY significantly reversed the TSH-induced decrease in IRS-1 tyrosyl phosphorylation. 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Disease Markers

fig6

Figure 6

Figure 6: Thyroid-Stimulating Hormone Inhibits Insulin Receptor Substrate-1 Expression and Tyrosyl Phosphorylation in 3T3-L1 Adipocytes by Increasing NF-<i>κ</i>B DNA-Binding Activity 

Figure 6 | Thyroid-Stimulating Hormone Inhibits Insulin Receptor Substrate-1 Expression and Tyrosyl Phosphorylation in 3T3-L1 Adipocytes by Increasing NF-κB DNA-Binding Activity 