COPS3 Promotes Proliferation, Invasion, and EMT of Colorectal Cancer Cells by MEK/ERK Pathway

Figure 2

COPS3 ablation suppressed the viability of COAD cells and stimulated apoptosis. (a) qPCR assays showed the mRNA levels of COPS3 in SW480 and HCT116 cells upon the transfection of control or COPS3 siRNAs or without transfection (control). (b) Immunoblot showed the expression of COPS3 in SW480 and HCT116 cells upon the transfection of control or COPS3 siRNAs or without transfection (control). (c) CCK-8 assays showed the OD value at 450 nm wavelength of SW480 and HCT116 cells upon the transfection of control or COPS3 siRNAs or without transfection (control). (d and e). Colony formation assays showed the colony number of SW480 and HCT116 cells upon the transfection of control or COPS3 siRNAs or without transfection (control). The quantification was in panel (e). (f and g). Flow cytometry (FCM) assays showed the apoptosis percentage of SW480 and HCT116 cells upon the transfection of control or COPS3 siRNAs or without transfection (control). The quantification was in panel (g). (h) Immunoblot showed the expression of the indicated proteins in control or COPS3 siRNAs or without transfection (control). Data are presented as . , siCOPS3 vs. siControl.

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