IDH1 Mutation Induces HIF-1α and Confers Angiogenic Properties in Chondrosarcoma JJ012 Cells

Figure 1

Downregulation of HIF-1α target genes upon loss of IDH1^mut in JJ012 cells. (a) Immunoblot shows depletion of IDH1 protein in two IDH1^mut KO clones of JJ012 cells. (b) HPLC-MS analysis indicates D-2HG production was nearly depleted in two IDH1^mut KO clones of JJ012 cells. (c) Heatmaps of HIF-1α target genes that are downregulated in both IDH1^mut KO clones compared to parental control of JJ012 cells. (d) mRNA expression of VEGFA, VEGFC, EDN1, and SLC2A3 in JJ012 cells was quantified by qRT-PCR. The amount of transcript was normalized to GAPDH, and the results are shown as fold-change relative to the parental control. Data are shown as of triplicate values and are representative of three independent experiments. , , . (e) Immunoblot shows levels of wildtype IDH1 in CRISPR/Cas9 KO C28 chondrocytes. (f) Bar graphs compare levels of VEGFA, VEGFC, EDN1, and SLC2A3 mRNA expression in parental and wildtype IDH1 KO C28 cells. NS: nonsignificant ().

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