Investigation of the Potential Key Genes and the Multitarget Mechanisms of Polygonum cuspidatum against Heart Failure Based on Network Pharmacology and Experimental Validation

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Gene</td><td class="align_center">Forward primer (5<svg height="11.4859pt" id="M5" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg> to 3<span class="nowrap"><svg height="11.4859pt" id="M6" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>)</span></td><td class="align_center">Reverse primer (5<svg height="11.4859pt" id="M7" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg> to 3<span class="nowrap"><svg height="11.4859pt" id="M8" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>)</span></td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">TNF</td><td class="align_center">CCCTGGTATGAGCCCATCTATC</td><td class="align_center">AAAGTAGACCTGCCCAGACTCG</td></tr><tr><td class="align_left">NFKB1</td><td class="align_center">ACACCGTGTAAACCAAAGCC</td><td class="align_center">CAGCCAGTGTTGTGATTGCT</td></tr><tr><td class="align_left">FOXO3</td><td class="align_center">CAGATCTACGAGTGGATGGTG</td><td class="align_center">TCTTGCCAGTTCCCTCATTC</td></tr><tr><td class="align_left">SOD1</td><td class="align_center">CCGGCTTGTCTGATGGAGAT</td><td class="align_center">TGCATCTTTTGGTCCACCGT</td></tr><tr><td class="align_left">GPX1</td><td class="align_center">TGAGAAGTGCGAGGTGAATG</td><td class="align_center">CGGGGACCAAATGATGTACT</td></tr><tr><td class="align_left">GAPDH</td><td class="align_center">TGGTGGACCTCATGGCCTAC</td><td class="align_center">CAGCAACTGAGGGCCTCTCT</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Sequences of primers used quantitative real-time PCR.</div>

Disease Markers

tab1

Table 1

Table 1: Investigation of the Potential Key Genes and the Multitarget Mechanisms of Polygonum cuspidatum against Heart Failure Based on Network Pharmacology and Experimental Validation 

Table 1 | Investigation of the Potential Key Genes and the Multitarget Mechanisms of Polygonum cuspidatum against Heart Failure Based on Network Pharmacology and Experimental Validation 