Hsa_circ_0097271 Knockdown Attenuates Osteosarcoma Progression via Regulating miR-640/MCAM Pathway

Figure 6

MCAM was a downstream target of miR-640. (a) The predicted binding site between miR-640 and MCAM 3’UTR was obtained from TargetScan. (b) The binding site between miR-640 and MCAM was verified by dual-luciferase reporter assay, relative to miR-NC. (c) MiR-640 expression in OS tumor tissues and normal controls was checked by qPCR. (d) MiR-640 expression in OS cells (Saos-2 and SW1353) and normal cells (hFOB1.19) was checked by qPCR, relative to hFOB1.19. (e) The linear relationship between miR-640 expression and MCAM expression in OS tissues was analyzed by Pearson’s analysis. (f) The linear relationship between circ_0097271 expression and MCAM expression in OS tissues was analyzed by Pearson’s analysis. (g) The expression of MCAM mRNA in si-circ or si-NC transfected Saos-2 and SW1353 cells was investigated by qPCR. relative to si-NC. (h) The expression of MCAM protein in si-circ or si-NC transfected Saos-2 and SW1353 cells was investigated by western blotting. relative to si-NC.

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