Cancer-Associated Exosomal CBFB Facilitates the Aggressive Phenotype, Evasion of Oxidative Stress, and Preferential Predisposition to Bone Prometastatic Factor of Breast Cancer Progression

Figure 2

CBFB-rich exosomes promoted metastatic potential in vitro. (a) Exosomes from bone metastatic patients exhibited significantly higher CBFB levels than those from primary and control patient counterparts. CD63 and CD9 was assessed to confirm the existence of exosomes. Coculture of serum exosomes from bone metastatic patients significantly increased the migration (b) and invasion (c) abilities of low-metastatic-potential breast cancer cell lines MCF12A and T47D. Representative images of invasion are shown. Scale bars: 200 μm. (d) Analytical qPCR and Western blotting results demonstrated that exosome-treated MCF12A and T47D cells expressed a markedly higher level of Snail, CD44, OPN, CBFB, and Runx2 compared with their controls. NS: not significant; , .

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