Cancer-Associated Exosomal CBFB Facilitates the Aggressive Phenotype, Evasion of Oxidative Stress, and Preferential Predisposition to Bone Prometastatic Factor of Breast Cancer Progression

Figure 4

CBFB expression was associated with oxidative stress and metastatic potential of breast cancer cells. (a) Western blot images indicating the effect of CBFB-knockdown on the expression of CBFB, NAE1, NFATC3, NOS2, and PARP1 in highly metastatic MDA-MB-436 cells. (b) CBFB-knockdown MDA-MB-436 cells (sh) exhibited significantly reduced migration (left panels) and invasion (right panels) abilities. The insert represents the Western blots indicating CBFB was silenced compared with negative control (NC). (c) Western blots demonstrating the significantly reduced expression of Snail, vimentin (Vim), OPN, CD44, and Runx2 in CBFB-knockdown MDA-MB-436 cells compared with their parental counterparts (NC). (d) Western blot images indicating the effect of CBFB-knockdown on the expression of CBFB, NAE1, NFATC3, and NOS2 in nonmetastatic T47D cells overexpressed with CBFB. (e) CBFG-overexpressing (OE) T47D cells exhibited enhanced migration (left panels) and invasion (right panels) abilities compared with their parental counterparts (NC). Representative images of invasion are shown. Scale bars: 200 μm. (f) Western blots comparing parental and CBFB-overexpressing T47D cells. Metastasis-associated markers Snail, CD44, Vim, OPN, and Runx2 were all elevated in the OE cells. (g) Tumorsphere formation from MDA-MB-436 and T47D. (h) CO-IP experiment checks the interaction between factors affected after altering CBFB expression, . β-Actin and GAPDH are the loading control.

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