Cancer-Associated Exosomal CBFB Facilitates the Aggressive Phenotype, Evasion of Oxidative Stress, and Preferential Predisposition to Bone Prometastatic Factor of Breast Cancer Progression

Figure 5

Downregulation of CBFB led to decreased bone metastasis in vivo. (a) Graph of tumor size over time indicating that the tumor burden of the CBFB-knockdown group was significantly lower than that of their control counterparts, . (b) Kaplan–Meier survival curve indicating that CBFB-knockdown mice exhibited a significantly higher percentage survival than their control counterparts. (c) IHC images of the differential expression of CBFB, OPN Runx2, and CXCR4 protein levels of the control and CBFB-knockdown mice. (d) CBFB level in the exosomes isolated from the pooled serum of CBFB-knockdown mice was significantly lower than that of their control counterparts. (e) qPCR examination of bone metastasis-associated circulating markers in the serum of both control and CBFB-knockdown mice. CBFB-knockdown tumor samples exhibited a significantly lower level of CXCR4, OPN, Runx2, and IL-6 than did their control counterpart. , .

(a)

(b)

(c)

(d)

(e)