Inhibition of miR-let-7i Induces DC Immature Cells and Improves Skin Graft Tolerance

Figure 5

ImDCs transfected with the miR-let-7i inhibitor inhibited allograft rejection. (a–e) Western blot experiment to detect the production of IL-2, IL-4, IL-10, and IFN-γ. (f) RT–qPCR to detect the expression of miR-let-7i. (g) RT–qPCR to detect the expression of IL-2, IL-4, IL-10, and IFN-γ (upper: 5 days and lower: 10 days). (h and i) HE detects the condition of transplanted skin tissue. Each experiment was repeated at least 3 times for each sample, and 1-12 indicates the transplant site number, , , , and .

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