Tumor Suppressor Role and Clinical Significance of the FEV Gene in Prostate Cancer

Figure 3

FEV overexpression disrupts LNCaP cell proliferation, invasion, and migration yet promotes apoptosis in vitro. (a) Enforced expression of FEV in the FEV-stably transfected LNCaP cells verified by western blot and qRT-PCR. (b) Transwell analysis revealed that FEV overexpression suppresses the invasive ability of LNCaP cells. The results of the statistical analysis of three independent experiments are shown in panel (c). (d) Wound healing assays showed that FEV upregulation inhibits the migration of LNCaP cells. The results of statistical analysis of three independent experiments are shown in panel (e). (f) CCK-8 assays revealed that FEV overexpression disrupts the proliferation of LNCaP cells. (g) Enforced expression of FEV-induced apoptosis of LNCaP cells. The results of statistical analysis of three independent experiments are shown in the panel. (h) Enforced expression of FEV inhibits the cell cycle of LNCaP cells. Data were presented as the . , relative to the negative control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)