Development of a Costimulatory Molecule Signature to Predict Prognosis, Immune Landscape, and Response to Immune Therapy for Hepatocellular Carcinoma

Figure 9

TNFRSF4 was an oncogene in HCC. (a) TNFRSF4 was highly expressed in tumor than normal specimen. (b) The TNFRSF4 expression level in human normal liver and HCC cell lines with qRT-PCR. (c) Validation of siRNA knockdown efficiency in HuH7 and Li-7 cells by qRT-PCR. (d, e) Cell viability of HuH7 and Li-7 cells after knocking down TNFRSF4 was detected using CCK-8 assay and colony assay. (f) Flow cytometry analysis of apoptosis in siNC and siTNFRSF4 transfected HuH7 and Li-7 cells. (g) The relative protein expression of apoptosis was determined in TNFRSF4-knockdown HuH7 cells by using western blot analysis.

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