Triptolide Inhibits the Biological Processes of HUVECs and HepG2 Cells via the Serine Palmitoyltransferase Long Chain Base Subunit 2/Sphingosine-1-Phosphate Signaling Pathway

Figure 2

TP significantly downregulated the expression of SPTLC2 in HUVECs. (a) HUVECs were treated with TP (25 nM) for 24 h, and the changes in related genes were screened by PCR array assay. The results are shown as the absolute value of . (b) HUVECs were treated with TP (25 nM) for 24 h, and the expression of SPTLC2 mRNA was detected by RT-PCR assay. (c) HUVECs were treated with TP (0, 12.5, 25, or 50 nM) and DMSO (negative control) for 24 h, and the protein expression of SPTLC2 in HUVECs was detected by western blotting assay. , versus the control group.

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